The Blog on Biosafety Cabinets
Enhancing Laboratory Safety with Class 2 Biosafety Cabinets and Microbiological Protection
In today’s scientific and clinical labs, safety for personnel and samples is non-negotiable. Whether it's biotech development, pharmaceutical research, or microbiological analysis, biosafety cabinets form the backbone of contamination control.
Of all types, Class 2 cabinets are most commonly used due to their efficiency and versatility. These units are crucial for working with moderate-risk organisms and sensitive samples.
Biosafety Cabinets – How They Work and Why They Matter
Biosafety cabinets are airflow-controlled workspaces engineered for microbiological safety. They use high-efficiency filters to purify air entering and leaving the cabinet.
These units are generally classified into Classes I, II, and III depending on airflow and application needs. Among these, Class 2 cabinets are the most versatile and commonly used.
Defining Class 2 Biosafety Cabinets and Their Role
Class 2 Biosafety Cabinets ensure safety on all fronts—personnel, workspace, and biological material. They utilise downward laminar airflow within a sealed system.
Both incoming and outgoing air are HEPA-filtered to maintain sterility and prevent leaks. These cabinets are frequently used in labs handling infectious agents or clinical samples.
What Makes Class 2 Cabinets Effective in Labs
A Class 2 microbiological safety cabinet includes several integrated safety mechanisms such as:
• Medical-grade filters for capturing contaminants
• Steady air movement to reduce airborne particle circulation
• Negative pressure barriers to prevent leakage
• Optional UV lights for overnight sterilisation
• Low sound emissions to reduce fatigue
• Front glass for full control and protection
These elements create a clean, contained, and user-friendly workspace.
Where Class 2 Cabinets Are Used
Class 2 Biosafety Cabinets are commonly used across microbiology, biotechnology, diagnostics, and pharma. They are critical for DNA/RNA extraction, pathogen culture, and molecular assays.
From universities to private pathology labs, Class 2 cabinets ensure lab hygiene and sample integrity.
Why Laboratories Prefer Class II Safety Cabinets
Using Class 2 cabinets offers significant improvements in contamination control and user safety:
• Prevents contamination during sensitive procedures
• Acts as a barrier between user and biohazard
• Minimises lab contamination and pollution risks
These cabinets combine safety features with operational efficiency.
Design and Compliance Standards
Top manufacturers ensure their cabinets meet certifications like NSF 49, EN 12469, and WHO recommendations. Class 2 units are sub-classified as A1, A2, B1, and B2—based on varying airflow balance and ducting needs.
• Type A2: Recirculates 70% and exhausts 30% of filtered air
• Type B2: Used for hazardous vapors and chemicals
Matching the cabinet type to your process is essential.
Tips for Purchasing the Ideal Biosafety Cabinet
Before purchasing, consider:
• Your application type (e.g., diagnostics, pharma, research)
• Available lab space and utility infrastructure
• Ease of use, energy efficiency, and upkeep
• Warranty, training, and certification services
Partnering with a trusted provider helps meet both performance and safety needs.
Safe Setup and Operation of Class 2 Cabinets
For optimal results:
• Avoid placing near doors, vents, or fans
• Get periodic validation from certified technicians
• Educate staff on cabinet operations and safety
Operational best practices include:
• Always wear appropriate microbiological safety cabinet lab PPE
• Avoid sudden or quick arm movements
• Clean all contact points post-operation
• Treat UV usage as an overnight sterilisation method
Final Thoughts on Class 2 Biosafety Cabinets
Class 2 biosafety cabinets are vital equipment in laboratories dealing with biohazards. They protect workers, secure samples, and copyright environmental standards.
From pathology labs to advanced genetic testing, Class II cabinets maintain sterility in sensitive procedures. When investing in a biosafety cabinet, prioritise compliance, usability, and long-term support—because your research deserves the best protection.